Studies on the Inactivation of Catalase.” Ii. Inactivation by Ultra-violet Radiation at Different Hydrogen Ion Concentrations. by Sergius Morgulis
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چکیده
In a previous paper (1) the inactivation of catalase by heat at different pH was studied. It was discovered that the heat inactivation depends upon the hydrogen ion concentration of the enzyme solution which affects both the velocity and the extent of the reaction. It was also found there that 65” was the critical temperature for the enzyme preparation since at this temperature the catalase was instantly inactivated at all pH. It was further found that the degree of inactivation at different temperatures follows a rather characteristic curve, the catalase being most stable at pH 6.0 to 6.5 and losing its activity somewhat more rapidly on the acid than on the alkaline side of that pH. Catalase does not manifest a distinct optimum hydrogen ion concentration for its activity, this being covered by a range from about pH 6.5 to 8.5, so that the condition for the maximum stability of the enzyme towards heat does not exactly coincide with this range but falls only within its outside acid margin. This observation suggested that attempts to determine the chemical nature of the enzyme from its activity at different hydrogen ion concentrations must necessarily lead to erroneous conclusions and that the study of the
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Studies on the Inactivation of Catalase.” Ii. Inactivation by Ultra-violet Radiation at Different Hydrogen Ion Concentrations. by Sergius Morgulis
In a previous paper (1) the inactivation of catalase by heat at different pH was studied. It was discovered that the heat inactivation depends upon the hydrogen ion concentration of the enzyme solution which affects both the velocity and the extent of the reaction. It was also found there that 65” was the critical temperature for the enzyme preparation since at this temperature the catalase was...
متن کاملStudies on the Inactivation of Catalase.” Ii. Inactivation by Ultra-violet Radiation at Different Hydrogen Ion Concentrations. by Sergius Morgulis
In a previous paper (1) the inactivation of catalase by heat at different pH was studied. It was discovered that the heat inactivation depends upon the hydrogen ion concentration of the enzyme solution which affects both the velocity and the extent of the reaction. It was also found there that 65” was the critical temperature for the enzyme preparation since at this temperature the catalase was...
متن کاملStudies on the Inactivation of Catalase.” Ii. Inactivation by Ultra-violet Radiation at Different Hydrogen Ion Concentrations. by Sergius Morgulis
In a previous paper (1) the inactivation of catalase by heat at different pH was studied. It was discovered that the heat inactivation depends upon the hydrogen ion concentration of the enzyme solution which affects both the velocity and the extent of the reaction. It was also found there that 65” was the critical temperature for the enzyme preparation since at this temperature the catalase was...
متن کاملStudies on the Inactivation of Catalase Iii. Destruction of Catalase by Hydrogen Peroxide by Sergius Morgulis
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Studies on the Effect of Temperature on the Catalase Reaction. Vi. Heat Inactivation of Catalase at Different Hydrogen Ion Concentrations. by Sergius Morgulis
The method of experimentation in the present investigation has been improved in several essential points as compared to the original procedure. To avoid the undeterminable loss of activity incident to the warming of the enzyme solution to the desired temperature, a definite amount of a buffer mixture of known pH was heated to about 2” above the desired temperature. A measured quantity of enzyme...
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